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phosphoerk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphoerk
    Phosphoerk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+phosphoerk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Rabbit+mAb/pm41677613-95-34-36
    Average 96 stars, based on 1309 article reviews
    phosphoerk - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: Neuronal hyperexcitability: A key to unraveling hippocampal synaptic dysfunction in Lafora disease.
    Article Snippet: Tissues were homogenized at 4°C in ice- cold buffer containing .32 M sucrose, .1 mM phenylmethylulfonyl fluoride (PMSF), 1 mM HEPES, 1 mM MgCl, and 1 mM NaF, protease inhibitors (Complete, Sigma- Aldrich), and phosphatase inhibitors (PhosSTOP, Sigma- Aldrich). .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense | 5COSTA et al. 1:1000, #14544 s, Cell Signaling), rabbit anti- ERK (WB 1:1000, #9102, Cell Signaling), rabbit anti- phosphoERK (WB 1:1000, #9101, Cell Signaling), and mouse antitubulin (WB 1:10000, #T9026, Sigma- Aldrich). .. Membranes were blocked for 1 h at room temperature (I- block, TBS 1X, 20% Tween 20), and then incubated overnight at 4°C with primary antibodies: rabbit anti- GluA1 (WB 1:1000, #13185S, Cell Signaling), mouse anti- GluA2 (WB 1:2000, #75- 002, Neuromab), mouse anti- GluA3 (WB 1:1000, #MAB5416, Millipore), rabbit anti- phosphoSer845- GluA1 (WB 1:1000, #ab76321, Abcam), rabbit anti- GluN2A (WB 1:1000, #M264, Sigma- Aldrich), rabbit anti- GluN2B (WB 15281167, 0, guyen T am - T aipei M edical U niversity(T m u) , on [20/12/2025]

    Article Title: Neuronal Hyperexcitability: A Key to Unravelling Hippocampal Synaptic Dysfunctions in Lafora Disease
    Article Snippet: .. The following primary antibodies were used: rabbit anti-GluA1 (WB 1:1000, #13185S, Cell Signaling, Danvers, MA, USA), mouse anti-GluA2 (WB 1:2000, #75-002, Neuromab, UC Davis, Davis CA), mouse anti-GluA3 (WB 1:1000, #MAB5416, Millipore, Millipore, Burlington, MA, USA), monoclonal rabbit anti-phosphoSer845-GluA1 (WB 1:1000, #ab76321,Abcam), rabbit anti-GluN2A (WB 1:1000, #M264, Sigma-Aldrich), rabbit anti-GluN2B (WB 1:1000, #14544s, Cell signaling, Danvers, MA, USA), rabbit anti-ERK (WB 1:1000, #9102, Cell Signaling, Danvers, MA, USA), rabbit anti-phosphoERK (WB, 1:1000, #9101, Cell Signaling, Danvers, MA, USA) mouse anti-tubulin (WB 1:10000, #T9026, Sigma-Aldrich). .. The following secondary antibodies were used for WB analysis: goat anti-rabbit HRP and goat anti-mouse HRP (#1706515 and #1706516, respectively, Bio-Rad).

    Blocking Assay:

    Article Title: Engineering Tools to Study and Control Signaling in Neural Stem Cells
    Article Snippet: Blots were blocked for 1 h in tris-buffered saline (TBS) with 0.1% Tween-20 (TBS-T) and 3% bovine serum albumin (BSA) for phoshoprotein antibodies or 5% Blotting Grade Blocker (BioRad) for all other antibodies. .. Blots were probed overnight at 4°C with primary antibodies in the same blocking buffer: rabbit anti-Ack1 (1:1000, PA5-14765, ThermoFisher Scientific), rabbit anti-phospho-Ack1 (1:500, PA5-37782, ThermoFisher Scientific), rabbit anti-phospho-Akt (1:2000, 4060, Cell Signaling), rabbit anti-β-catenin (1:1000, 9562, Cell Signaling), rabbit antinon-phospho (Active)-β-catenin (1:1000, 8814, Cell Signaling), goat anti-EphB4 (1:1000, AF446, R&D Systems), rabbit anti-ERK (1:1000, 4695, Cell Signaling), rabbit anti-phosphoERK (1:2000, 4370, Cell Signaling), rabbit anti-FAK (1:1000, 3285, Cell Signaling), rabbit antiphospho-FAK (1:1000, ab81298, Abcam), rabbit anti-Fyn (1:1000, ab125016, Abcam), rabbit anti-phospho-Fyn (1:1000, SAB4503872, Sigma-Aldrich), rabbit anti-GAPDH (1:2500, ab9485, Abcam), rabbit anti-phospho-MEK (1:1000, 9154, Cell Signaling), rabbit anti-phosphoserine (1:100, ab9332, Abcam), rabbit anti-phosphothreonine (1:1000, 9381, Cell Signaling), mouse anti-phosphotyrosine (1:1000, 9411, Cell Signaling), rabbit anti-phospho-PI3K (1:1000, 4228, Cell Signaling), mouse anti-Src (1:1000, 05-184, Millipore), rabbit anti-phospho-Src (1:1000, 2101, Cell Signaling); followed by 1 h incubation with appropriate horseradish peroxidaseconjugated secondary antibodies: mouse anti-goat HRP (1:5,000, 31400), goat anti-mouse HRP (1:10,000, 32430), goat anti-rabbit HRP (1:10,000, 32460), all from ThermoFisher Scientific. .. Protein bands were detected using SuperSignal West Dura Chemiluminescent Substrate (ThermoFisher Scientific), and blots were digitally imaged on a ChemiDoc XRS+ Imaging System.

    Article Title: ISL1-overexpressing BMSCs attenuate renal ischemia-reperfusion injury by suppressing apoptosis and oxidative stress through the paracrine action.
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA, Gibco, USA) for 1 h at room temperature, membranes were incubated with primary antibodies, including rabbit anti-ISL1 (1:5000, Novus Biologicals, Colorado, USA, #NBP2-14999), mouse anti-Haptoglobin (1:500, Santa Cruz Biotechnology, USA, #sc-376,893), mouse anti-Bcl-2 (1:1000, Cell Signaling Technology, USA, #15071S), rabbit anti-Bax (1:1000, Cell Signaling Technology, USA, #2772S), rabbit anti-ERK (1:1000, Cell Signaling Technology, USA, #4695S), rabbit anti-phosphoERK (1:1000, Cell Signaling Technology, USA, #4370S), mouse anti-JNK (1:500, Santa Cruz Biotechnology, USA, #sc-137,019), mouse anti- phospho-JNK (1:500, Santa Cruz Biotechnology, USA, #sc-293,136) and mouse anti-GAPDH (1:5000, Proteintech, 60004-1-Ig) overnight at 4 °C. .. The membranes were washed with PBS containing Tween 20 and incubated with HRP-conjugated secondary antibodies (1: 5000, Abcam, Cambridge, UK) for 1 h at room temperature.

    Incubation:

    Article Title: Engineering Tools to Study and Control Signaling in Neural Stem Cells
    Article Snippet: Blots were blocked for 1 h in tris-buffered saline (TBS) with 0.1% Tween-20 (TBS-T) and 3% bovine serum albumin (BSA) for phoshoprotein antibodies or 5% Blotting Grade Blocker (BioRad) for all other antibodies. .. Blots were probed overnight at 4°C with primary antibodies in the same blocking buffer: rabbit anti-Ack1 (1:1000, PA5-14765, ThermoFisher Scientific), rabbit anti-phospho-Ack1 (1:500, PA5-37782, ThermoFisher Scientific), rabbit anti-phospho-Akt (1:2000, 4060, Cell Signaling), rabbit anti-β-catenin (1:1000, 9562, Cell Signaling), rabbit antinon-phospho (Active)-β-catenin (1:1000, 8814, Cell Signaling), goat anti-EphB4 (1:1000, AF446, R&D Systems), rabbit anti-ERK (1:1000, 4695, Cell Signaling), rabbit anti-phosphoERK (1:2000, 4370, Cell Signaling), rabbit anti-FAK (1:1000, 3285, Cell Signaling), rabbit antiphospho-FAK (1:1000, ab81298, Abcam), rabbit anti-Fyn (1:1000, ab125016, Abcam), rabbit anti-phospho-Fyn (1:1000, SAB4503872, Sigma-Aldrich), rabbit anti-GAPDH (1:2500, ab9485, Abcam), rabbit anti-phospho-MEK (1:1000, 9154, Cell Signaling), rabbit anti-phosphoserine (1:100, ab9332, Abcam), rabbit anti-phosphothreonine (1:1000, 9381, Cell Signaling), mouse anti-phosphotyrosine (1:1000, 9411, Cell Signaling), rabbit anti-phospho-PI3K (1:1000, 4228, Cell Signaling), mouse anti-Src (1:1000, 05-184, Millipore), rabbit anti-phospho-Src (1:1000, 2101, Cell Signaling); followed by 1 h incubation with appropriate horseradish peroxidaseconjugated secondary antibodies: mouse anti-goat HRP (1:5,000, 31400), goat anti-mouse HRP (1:10,000, 32430), goat anti-rabbit HRP (1:10,000, 32460), all from ThermoFisher Scientific. .. Protein bands were detected using SuperSignal West Dura Chemiluminescent Substrate (ThermoFisher Scientific), and blots were digitally imaged on a ChemiDoc XRS+ Imaging System.

    Article Title: ISL1-overexpressing BMSCs attenuate renal ischemia-reperfusion injury by suppressing apoptosis and oxidative stress through the paracrine action.
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA, Gibco, USA) for 1 h at room temperature, membranes were incubated with primary antibodies, including rabbit anti-ISL1 (1:5000, Novus Biologicals, Colorado, USA, #NBP2-14999), mouse anti-Haptoglobin (1:500, Santa Cruz Biotechnology, USA, #sc-376,893), mouse anti-Bcl-2 (1:1000, Cell Signaling Technology, USA, #15071S), rabbit anti-Bax (1:1000, Cell Signaling Technology, USA, #2772S), rabbit anti-ERK (1:1000, Cell Signaling Technology, USA, #4695S), rabbit anti-phosphoERK (1:1000, Cell Signaling Technology, USA, #4370S), mouse anti-JNK (1:500, Santa Cruz Biotechnology, USA, #sc-137,019), mouse anti- phospho-JNK (1:500, Santa Cruz Biotechnology, USA, #sc-293,136) and mouse anti-GAPDH (1:5000, Proteintech, 60004-1-Ig) overnight at 4 °C. .. The membranes were washed with PBS containing Tween 20 and incubated with HRP-conjugated secondary antibodies (1: 5000, Abcam, Cambridge, UK) for 1 h at room temperature.

    Immunostaining:

    Article Title: Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling.
    Article Snippet: Article Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling

    Labeling:

    Article Title: Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling.
    Article Snippet: Article Regionally mapped astrocytic responses to cortical and white matter stroke show differential roles in astrocyte-induced vascular remodeling

    other:

    Article Title: Palmitoylation Couples DLK to JNK3 to Facilitate Pro-degenerative Axon-to-Soma Signaling
    Article Snippet: Primary antibodies used were as follows: Rabbit anti-phospho c-Jun S63 (#91952, and rabbit anti-phospho c-Jun S63 II (Cell Signaling Technology, #9261, both used for DRG neuron experiments), Rabbit anti-phospho c-Jun Ser-73 (Cell Signaling Technology, #3270, used for RGC experiments); rabbit anti-DLK/MAP3K12 (Sigma/ Prestige, #HPA039936); mouse anti-GFP (Life Technologies, #A11120, clone 3E6 IgG2a), rabbit anti-GFP (Life Technologies, #A11122), mouse anti-Tubulin β3 (BioLegend, TUJ1, Ig2a, Covance catalog# MMS-435P), Myc 9E10 (University of Pennsylvania Cell Center, Catalog #3207), mouse anti-Myc (Enzo, myc9E10), mouse anti-HA11 (BioLegend, 901502, IgG1), rabbit anti HA (Cell Signaling Technology, #3724), mouse anti-tubulin (Millipore Sigma, Catalog #T7451), sheep anti-NGF (CedarLane, catalog #CLMCNET-031), Rabbit anti-phosphoMKK4 (Cell Signaling Technology, #4514, Rabbit anti-MKK4 (Cell Signaling Technology, #9152), Rabbit anti-phosphoAkt T308 (Cell Signaling Technology, #9275, Rabbit anti-Akt (Cell Signaling Technology, #4691), Rabbit anti-phosphoERK (Cell Signaling Technology, #9106), mouse anti-panERK1/2 (Cell Signaling Technology, #9106), mouse anti-phosphoJNK (Cell Signaling Technology, #9255), rabbit anti pan-JNK (Santa Cruz Biotechnology, #SC-571), rabbit anti-phospho-MAPKAPK2 T334 (Cell Signaling Technology, #3041), rabbit anti-phospho-p38 MAPK Cell Signaling Technology, #4511).



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